proteinsimple jess protocol28 May proteinsimple jess protocol
Simple Western data are displayed by lane in a virtual blot-like image similar to traditional Western blot results. Bio-Techne uses cookies to provide you with a great website experience. 2. Validation was considered when differential expression at one or more . Research Fellow, Brain Research Institute Monash Sunway (BRIMS), "It saves so much time! In Vitro Technologies :: JESS Just load your samples and reagents into the microplate, and Jess does the rest. Just load your samples and reagents into the microplate, and Jess does the rest. Learn how various capillary electrophoresis techniques can be easily implemented along the development path to support gene therapy development. Jess automates traditional Western blotting while maximizing multiplexing with multiple detection channels. PDF Compass Software for Simple Western Size Assays Quick Reference Guide With proteins now immobilized and the matrix cleared of the capillary, Jess starts the immunoprobing process. Next, your protein lysate is loaded, and capillaries are lowered to make contact with running buffer. Insert a capillary cartridge into the cartridge holder. Which Brands are Currently Available on biotechne.com? ProteinSimple: Transferring your Traditional Western Blot to Wes For fluorescent detection, samples are incubated with the primary antibody followed by infrared or near-infrared fluorescent secondary tagged antibodies. % Learn about protocols for the Wes and Jess Simple Western instruments by ProteinSimple. USA, TEL 612 379 2956 The sensitivity of Simple Western Total Protein Assays can be adjusted based on the needs of your sample by adjusting the concentration of Biotin Labeling Reagent. Copyright 2023 Bio-Techne. Let your sample go further with Jess. R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through biotechne.com. This had previously taken weeks and months using traditional Western blotting methods", Krishna C. Yalla, Ph.D., Jess, she's like western blot meets ELISA in one. R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through biotechne.com. This RePlex Module provides all the reagents needed to remove primary and secondary antibodies in a RePlex assay with two immunoassays or immunoassay with Total Protein assay in a single run. Why bother stripping and reprobing? Processing time of 3 hours for 25 samples to dramatically speed up time to results compared to traditional Western blotting protocols. With Stellar modules, low abundance proteins can be detected and multiple targets can be multiplexed in the same sample using multi-color fluorescence channels. Or add total protein without giving up a detection channel. Catalog No. endobj Copyright 2023 R&D Systems, Inc. All Rights Reserved. Chemiluminescent Western blots imaged on Jess. Image traditional chemiluminescent blot membranes 2. Minneapolis, MN 55413 Simple Western Total Protein Assay | Bio-Techne For more information visit or contact us at: Toll-free: 888 607 9692 Tel: 408 510 5500 . Senior Research Scientist, TCG Lifesciences, "Jess allows me to reduce the preparation time, save on reagents, samples, and antibodies, while allowing easier optimization of target proteins/antibodies. ProteinSimple, the ProteinSimple logo, Jess and Simple Western are trademarks and/ ACD branded products will be available on biotechne.com in the near future. If Jess is damaged and doesn't function properly, stop the instrument safely and contact ProteinSimple Technical Support right away toll-free in the US and Canada at 1-888-607-9692 (option 3). Table of Contents: There are 3 main detection options available for total protein analysis on Simple Western : ", Chris LaBreck, Ph.D., Jess, shes like Western blot meets ELISA in one. While SYPRO Ruby requires at least 1 ng of protein for reliable detection, Simple Western Total Protein Assay Methodsusing 5X Biotin Labeling Reagent can reliably detect as little as 150 pg. PDF YOUR COMPLETE PROTEIN ANALYSIS SOLUTION - Biomed Global 2012 - 2020. Screen for processing-related impurities and host-cell related contaminants. Jess - Chemiluminescent & Fluorescent Western Blotting | ProteinSimple Automation of protein separation and immunodetection eliminates many of the tedious, error-prone steps of traditional Western blotting which limit data quality. Her in-capillary protein normalization reagent is an easy way to see if your samples contain a consistent protein load and investigate experimental setup and user errors. ProteinSimple will continue service and support of the Wes platform through July 30th, 2026. The Simple Western : a gel-free, blot-free, hands-free - Nature Senior Researcher, EURAC Research, "I work for a fast-paced drug discovery CRO where our clients expect high-quality data with rapid turnarounds. And with in-built protein normalization, you'll have the confidence you need in your results, experiment after experiment. WES and JESS: Automated Western Blotting - YouTube Or get info on protein abundance, isoform and size all at once. Side-by-side comparison of Simple Western Total Protein Assay for AAV Analysis using SYPRO Ruby (right) and 5X Biotin Labeling Reagent with Simple Western (left) shows that the Simple Western Total Protein Assay surpasses the sensitivity of traditional protein stains. Bio-Techne uses cookies to provide you with a great website experience. We only had a small number of [iPSC-derived macrophages] because the cell line does not proliferate. Analyzing Proteins with Jess. The chemiluminescent reaction is recorded by a CCD camera in a series of images over time. stream Faculty Fellow, Wolfson Childhood Cancer Research Centre, Northern Institute for Cancer Research, Newcastle University, "Wes allows us to measure cell signaling proteins from a single muscle fiber segment by lowering our limit of detection nearly ~20-fold from Western blotting. Plus, use the Stellar Total Protein assay to normalize your multiplexed . info@proteinsimple.com proteinsimple.com . Come back to fully analyzed and quantitated results in three hours. Simple Western can do the work for you. R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through biotechne.com. And obviously I like getting results the same day rather than having to wait two days", Emma Wilson (ne Humphrey), Ph.D., ProteinSimple will continue service and support of the Wes platform through July 30th, 2026. Forbes D. Porter. Postdoc, DIOSynVax, Department of Veterinary Medicine, Cambridge, United Kingdom, "Its had a significant impact on our research we get double the amount of data in a third of the time. We offer a 100% guarantee on all our products. Jess Simple Western System 004-650: R&D Systems Head of Research Instrumentation, Cancer Research UK Cambridge Institute, "Some of the proteins we wanted to detect are low in abundance and its really hard to get a good amount of protein for traditional Western blot. Find protocols for special samples as well as specific applications. Simply load your sample, antibodies and reagents into the plate, insert your plate and cartridge into Jess and press start. The detection of proteins from pairs of lens epithelial cell samples requires the ProteinSimple JESS or WES capillary electrophoresis machine (Parreno et al., 2020; Cheng et al., 2022). After separation & immobilization of your sample proteins, the Protein Normalization Assay Module for Simple Western uses a proprietary molecule to fluorescently label proteins in your sample for detection. ", Melyssa Bratton, Ph.D., This RePlex Module provides all the reagents needed to remove primary and secondary antibodies in a RePlex assay with two immunoassays or immunoassay with Total Protein assay in a single run. Stellar NIR / IR modules for Jess set the standard for fluorescence Western blotting detection sensitivity. R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through biotechne.com. Which Brands are Currently Available on biotechne.com? ", Kazuya Machida, M.D., Ph.D., ", Miranda Orr, Ph.D., ACD branded products will be available on biotechne.com in the near future. Jess can dramatically increase your throughput. Quantify empty/full capsid content of viral vectors like AAVs. This allows us to use more advanced statistical techniques such as principal component analysis and hierarchal clustering to recognize key correlations in our research with ease", John K. Blackwood PhD, Associate Professor, Department of Physiology and Cell Biology, University of Nevada School of Medicine, "The traditional blotting method can use up to 300 pieces of plastic and [Simple Western] uses two. The variability in the manual processes that impact reproducibility, quantitation, time to result, and overall data reliability is eliminated. At best, you end up with Western blot-like, semi-quantitative results when what you really need, and deserve, is highly reproducible immunoassay quantitation. For samples containing 0.005 - 0.2 mg/mL of protein, chemiluminescence total protein assay methods can get you accurate normalized immunoassay data without having to rely on variable housekeeping proteins. Store the unopened product at room temperature. $141.48 / Each of 1. Formaldehyde fixation, C Fox, F Johnson, J Whiting and P Roller, Journal of Histochemistry and Cytochemistry, 1985; 33:845-853. Once the separation is complete, UV light immobilizes the proteins to the capillary wall. Boost your screening studies using high throughput & hands-free Simple Western systems for quantitative analysis. There are 3 main detection options available for total protein analysison Simple Western: Total protein normalization is a technique where target protein abundance is normalized to the overall amount of protein present in a sample rather than a housekeeping protein to minimize the impact of varying expression of a loading control. Novus Biologicals branded products are not currently available on biotechne.com and can be found at novusbio.com. ProteinSimple's Simple Western Jess, Abby and Wes Protocols - Bio-Techne Minneapolis, MN 55413 Our Wes runs are completed in less than 4 hours, as opposed to 4 days with traditional methods. Jess reagents and consumables from ProteinSimple, a Bio-Techne brand, are designed for use with ProteinSimple's Jess, a Simple Western platform, and must be used in conjunction with Compass Software version 2.5 or higher and compatible firmware. Custom Targeted Protein Degradation Services, Certificate of Analysis (COA) Finder Tool. Simple Western assays on Jess using RePlex reduce reagent and consumable costs. ", Mary G. Johlfs, M.S., Help scientists and fund you research. Postdoctoral Researcher, CSU Fullerton, "[Wes] Provides quantitative data, saves time and uses far less sample. Associate Professor, Department of Genetics and Genome Sciences, UConn Health, "Weve been able to increase productivity dramatically with Wes. Snap! Somebody have experience with the WES proteinsimple system? Everything happens inside a capillary, and Jess precisely controls the protein separation and reagent additions and incubations, even the detection. We will not share your information for any other purposes. ", Joseph Klebba, Ph.D., Jess - Chemiluminescent & Fluorescent Western Blotting | ProteinSimple Custom Targeted Protein Degradation Services, Certificate of Analysis (COA) Finder Tool, Chemiluminescent Total Protein Assay Methods, Download our white paper tolearn moreaboutSimple Western publication-ready results, Stellar Total Protein Detection Module (DMTP03), Simple Western Total Protein Assay Methods, Download the Total Protein Assay for AAV Analysis App Note, Simple Western Total Protein Assay for AAV Analysis, accurate normalized immunoassay data without having to rely on variable housekeeping proteins, Fluorescent Protein Normalization Assay Module, Fluorescent Protein Normalization App Note. PROTEINSIMPLE JESS USER MANUAL Pdf Download | ManualsLib Multiplexing takes your options to the max, helping you identify and differentiate relative protein expression between samples in one shot. Jess reagents and consumables from ProteinSimple, a Bio-Techne brand, are designed for use with ProteinSimple's Jess, a Simple Westernplatform, and must be used in conjunction with Compass Software version 2.5 or higher and compatible firmware. Lecturer in Molecular Biology, Institute of Medicine, University of Chester, "The no-gel, no-transfer, no-membrane features are beneficial for the reproducibility of the assay. Get the latest news, product updates, and promotions: The product is shipped at ambient temperature. All Rights Reserved. Upgrade to Jess or Abby to have all the functionality of Wes with additional features like RePlex and Stellar High Sensitivity NIR/IR Detection (Jess only). Remove the assay plate lid. Next-generation analytical instrumentation to help improve and accelerate your cell and gene therapy workflows. Senior Lecturer in Drug Action, Neuroscience, University of Manchester, "One of the best advantages of using Wes is that it saves a lot of time, both in terms of hands-on time and in time to completion of the experiment. Easy to use, very short learning curve. Jess automates the protein separation and immunodetection of traditional Western blotting, eliminating many of the tedious, error-prone steps. Jess Fully Automated Western System from ProteinSimple In contrast to membrane-anchored ADAMs, ADAMTSs are secreted and are all active proteases. Finland - (English) Country/Region selector. Jess offers market-leading protein detection sensitivity for Western blotting workflows. ProteinSimple Jess User Manual | Manualzz endobj 2. Select assay parameters in Compass software. FAX 612 656 4400. A validated workflow for protein analysis in extracellular vesicle samples from the leaders in exosome isolation and high sensitivity Western blotting solutions. ", Nicolas G. Bazan, MD, PhD, USA, TEL 612 379 2956 Best of all, you get reliable and reproducible results with target normalization to the amount of protein loaded. Get the picture with Jesss blot imaging system. ", Jane Gray, Ph.D., Jess allows me to accurately assess drug compound potency, and with its high throughput ability I can screen multiple compounds quickly and efficiently. Jess begins by aspirating the separation matrix and then the stacking matrix into each capillary. (Based on 1 Review), $25/18/15/$25CAN/75 Yuan/1250 Yen for a review with an image, $10/7/6/$10 CAD/70 Yuan/1110 Yen for a review without an image. Toll-free 800 343 7475 New Assay>Jess-Immunoassay: Fluorescence; Size Range:12-230 kDa; Cartridge: 25. Once the separation is complete, UV light immobilizes the proteins to the capillary wall. In just 3 hours you'll have quantitative, size-based data ready for analysis. CST Antibodies Validated for Simple Western Download our white paper tolearn moreaboutSimple Western publication-ready results. Which Brands are Currently Available on biotechne.com? All Rights Reserved. Even better, she'll quantitate the data for you too. Multiple with chemluminescence & incandescence canal. Just load your samples and reagents into the microplate and Jess does the rest. endobj Total protein detection happens in the same capillary on the same sample, but runs on a separate channel from NIR so you get to keep your existing IR, NIR and chemiluminescence channels. To gain the most data out of your precious sample, Jess provides a two-step immunoassay that is performed within the same capillary. PDF Analyzing Formalin-Fixed, Paran-Embedded Tissue on Simple Western Bio-Techne Brands Which Brands are Currently Available on biotechne.com? Decreasing concentrations of recombinant DnaK (15 g/mL, 7.5g/mL, and 3.75 g/mL) in HeLa whole-cell lysate and negative controls. Use your total protein assay data to identify impurities or contaminants in your sample or normalize your immunoassay data with total protein measurements for more reliable results. Copyright 2023 Bio-Techne. Jesss ability to detect multiple proteins per sample further saves time compared to conventional Western blot. Meet Jess, your protein analysis problem solver. Jess gives you four ways to analyze proteins. FAX 612 656 4400. Jess - Chemiluminescent & Fluorescent Western Blotting | ProteinSimple YOUR COMPLETE PROTEIN ANALYSIS SOLUTION - Bio-Techne Best of all, the transition is simplethe same antibodies you use for Western blotting protocols can be used with Simple Western assays. Meet Jess, your protein analysis problem solver. You'll be able to publish your results with confidence with effective normalization of your target protein expression, and if you're still doing traditional westerns, snap" get the picture with Jess's inbuilt plot imaging system. Custom Services for Cell & Gene Therapies, Good Manufacturing Practice (GMP) Antibody Services, View all Automated Western Blot Instrumentation FAQs. Professor and Chair, University of Birmingham, UK, "I was able to screen various antibodies in a single run within half a day. Setting the standard in quality research reagents for over 30 years, A trusted leader in quality life science reagents, Your trusted supplier for innovative and high performance life science reagents, Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis, Proprietary RNAscope technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity, A world leader in developing liquid biopsy based diagnostics, 614 McKinley Place NE Insert a capillary cartridge into the cartridge holder. Bio-Techne uses cookies to provide you with a great website experience. With Jesss chemiluminescent detection youll get picogram-level sensitivity, letting you maximize the data you get from your sample. For chemiluminescent detection, samples are first incubated with the primary antibody, followed by a secondary HRP conjugate, and finally chemiluminescent substrate. With Jess's chemiluminescent detection, you'll get picogram level sensitivity letting you maximize the data you get from your sample. Nuffield Department of Medicine, Target Discovery Institute, "Protein analysis using [Simple Western] can be performed faster, eliminating tedious work and saving time. Minneapolis, MN 55413 Place the assay plate on the plate . Just load your samples and reagents into the microplate, and Jess does the rest. Has anyone used the ProteinSimple Wes capillary western - ResearchGate Jess Reagents and Consumables | ProteinSimple - Bio-Techne Protein Simple- Jess. Or look at multiple targets and parameters simultaneously. Senior Research Scientist, Aurelia Bioscience, Biocity, Nottingham, UK, "The quantitative assays and short run times on Jess enable us to generate data faster and be more confident in our results. Improve reproducibility, quantitation, and time to market for your vaccine candidates. Jess automates the protein separation and immunodetection of traditional Western blotting, eliminating many of the tedious, error-prone steps. Simple Western immunoassays take place in a capillary. PDF Let's get started! - .NET Framework Open Compass software v6.0 or higher (Jess, Abby, or Wes) or Compass 5.0 (NanoPro 1000, Peggy Sue, Sally Sue) and click on the Assay icon. YOUR COMPLETE PROTEIN ANALYSIS SOLUTION - ProteinSimple - MedicalExpo Mayo Clinic Has anyone used the ProteinSimple Wes capillary western blot system for brain tissue? View all citations for the Simple Western platform. ProteinSimple branded instruments are available to quote. Jess - Chemiluminescent and Fluorescent Western Blotting :: ProteinSimple She separates your protein by size and precisely manages antibody additions, incubations, washes, and even the detection steps. With Jesss chemiluminescent detection youll get picogram-level sensitivity, letting you maximize the data you get from your sample. Please note this module can only be used with Jess. ProteinSimple branded instruments are available to quote. Jess gives you four ways to analyze proteins. She separates your protein by size and precisely manages antibody additions, incubations, washes and even the detection steps. Copyright 2023 Bio-Techne. ACD branded products will be available on biotechne.com in the near future. We appreciate your feedback. Bio-Techne uses cookies to provide you with a great website experience. ", Jon Voutila, Ph.D., Bounce to main content . Just load your samples and reagents into the microplate, and Jess does the rest. Texas A&M Institute for Genome Sciences and Society. ProteinSimple has developed Simple Western assays for protein sizing and quantitative immunodetection as an alternative to traditional Western blot analysis. PDF Examining Exosomes on Simple Western - Bio-Techne Please note this module can only be used with Jess. She automates the protein separation and immunodetection of traditional Western blotting, giving you fully analyzed results in just 3 hours. With a few clicks you'll be analyzing immunoassay-like standard curves and precisely quantifying your protein. Postdoc Researcher, Department of Orthopedics and Trauma Surgery, Medical University of Vienna, "It can be difficult to optimize new antibodies, particularly for brain markers where you have a lot of background noise and low levels of your target. Submit new antibody validation data to the Simple Western antibody database and receive a free 8x25 separation module or detection module! At the end of the run, Simple Western data are processed automatically in Compass for Simple Western. You just load your samples and wait for results, and in the meantime, you can dedicate yourself to other activities. 20,000 Jess' same-time detection also gives you more consistent, reliable results than traditional assays without having to stain and wash a single blot! Got a lot of samples? ", Tonmoy Sarkar, Ph.D., Meet Jess, your protein analysis problem solver. ", Dr. Matteo Ferrari, Meet Jess, your protein analysis problem solver. Use your total protein assay data to identify impurities or contaminants in your sample or normalize your immunoassay data with total protein measurements for more reliable results. Scientist, Vividion Therapeutics, "I used Simple Western because the protein samples I had were small. -Actin (9) Protein and mRNA Quantification in Small Samples of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes in 96-Well Microplates Methods in Molecular Biology October 5, . Novus Biologicals branded products are not currently available on biotechne.com and can be found at novusbio.com. I need to do protein normalization PDF Related Consumables - ", Mary Howell, At the conclusion of your run, use the lane view option to compare band intensity or dive deep for fully quantitative analysis of protein size and concentration. <>/ExtGState<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 612 792] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> Early Development Scientist, Oxford Biomedica, "Jess allows us to immediately identify potential autophagy biomarkers as they are upregulated or downregulated in response to treatment. Jess automates the protein separation and immunodetection of traditional Western blotting, eliminating many of the tedious, error-prone steps. By continuing to use this website you acknowledge this and agree to our cookie policy. Jess from ProteinSimple, a Bio-Techne brand, automatizes this protein separation & immunodetection from traditional Western dabbing. However, we are committed to improving your shopping experience. Jess automates the protein separation and immunodetection of traditional Western blotting, eliminating many of the tedious, error-prone steps. 3 0 obj 2 0 obj With protein normalization, you can take your protein load comparison and transform your data to effectively normalize your samples increasing your confidence in your data interpretation. The interior light will change from orange to blue. Simple Western assays on Jess using RePlexreduce reagent and consumable costs. Western blotting using #Jess #proteinsimple #westernblotting - ResearchGate With protein normalization, you can take your protein load comparison and transform your data to effectively normalize your samples, increasing your confidence in your data interpretation. 1. ", Jamie Boisvenue, FAX 612 656 4400. What's even better is that [Simple Western] can run up to 40 proteins in three days. She separates your protein by size and precisely manages antibody additions, incubations, washes and even the detection steps. Toll-free 800 343 7475 Use Jess's fluorescent capabilities and multiplex your proteins for even higher throughput. Qty Check Availability. All Rights Reserved. Open Jess's door. Jess offers market-leading protein detection sensitivity for Western blotting workflows. Just load your samples and reagents into the microplate and Jess separates your proteins by size, and precisely manages antibody additions, incubations, washes and even the detection steps. Using 5 times more concentrated Biotin Labeling Reagent, total protein detection using Simple Western can surpass the sensitivity of SDS-PAGE total protein stains including SYPRO Ruby. Protein normalization has never been easier with Jess. For samples containing 0.2 1.2 mg/mL of protein, a fluorescent total protein assay can be run on Jess using the Fluorescent Protein Normalization Assay Module (see next section on Fluorescent Total Protein Assays). Reduce your lab time while accelerating your COVID19 research. Head of Biology, and Jakub Stefaniak PhD, Samsara Therapeutics, "The introduction of an Abby will enable us to answer important questions quicker, such as identifying the disease driving mechanism and molecules surrounding osteoarthritis. Laboratory Coordinator, Department of Internal Medicine, East Tennessee State University, "The rapid, reproducible results on small amounts of tissue/cell lysates have allowed me to generate a more thorough data set on additional proteins, samples, conditions and brain regions all in the same amount, or even less time, as traditional Western blot. Custom Targeted Protein Degradation Services, Certificate of Analysis (COA) Finder Tool, Improved Serum and Plasma Biomarker Detection with Secondary SA-HRP, Analyzing Laser Capture Microdissection (LCM)Samples with Simple Western, Immunoprecipitation (IP) and Analysis with Simple Western, Analyzing Formalin-Fixed, Paraffin-Embedded Tissue on Simple Western, Analyzing Integral Membrane Proteins with Simple Western, Residual Protein A for Bioprocessing Applications, High-Throughput Glycan Characterization Using Simple Western, Improved Multiplexing with Simple Western Using a 20X Rabbit HRP Conjugate.
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